TY  - JOUR
AU  - Lakaniemi, A.-M.
AU  - Hulatt, C.J.
AU  - Wakeman, K.D.
AU  - Thomas, D.N.
AU  - Puhakka, J.A.
PY  - 2012//
TI  - Eukaryotic and prokaryotic microbial communities during microalgal biomass production
T2  - Bioresour Technol
JO  - Bioresource Technology
SP  - 387
EP  - 393
VL  - 124
KW  - Bacteria/classification/genetics/metabolism
KW  - *Biomass
KW  - Electrophoresis
KW  - Polyacrylamide Gel
KW  - Eukaryotic Cells
KW  - Microalgae/*metabolism
KW  - Phylogeny
KW  - Polymerase Chain Reaction
KW  - Prokaryotic Cells
AB  - Eukaryotic and bacterial communities were characterized and quantified in microalgal photobioreactor cultures of freshwater Chlorella vulgaris and marine Dunaliella tertiolecta. The microalgae exhibited good growth, whilst both cultures contained diverse bacterial communities. Both cultures included Proteobacteria and Bacteroidetes, while C. vulgaris cultures also contained Actinobacteria. The bacterial genera present in the cultures were different due to different growth medium salinities and possibly different extracellular products. Bacterial community profiles were relatively stable in D. tertiolecta cultures but not in C. vulgaris cultures likely due to presence of ciliates (Colpoda sp.) in the latter. The presence of ciliates did not, however, cause decrease in total number of C. vulgaris or bacteria during 14 days of cultivation. Quantitative PCR (qPCR) reliably showed relative microalgal and bacterial cell numbers in the batch cultures with stable microbial communities, but was not effective when bacterial communities varied. Raw culture samples were successfully used as qPCR templates.
SN  - 0960-8524
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22995170
UR  - PM:22995170
N1  - PMID:22995170
ID  - Lakaniemi_etal2012
ER  -