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Author Nguyen, H.X.; Nekanti, U.; Haus, D.L.; Funes, G.; Moreno, D.; Kamei, N.; Cummings, B.J.; Anderson, A.J. url  doi
openurl 
  Title Induction of early neural precursors and derivation of tripotent neural stem cells from human pluripotent stem cells under xeno-free conditions Type Journal Article
  Year 2014 Publication The Journal of Comparative Neurology Abbreviated Journal J Comp Neurol  
  Volume (down) 522 Issue 12 Pages 2767-2783  
  Keywords Cell Adhesion/drug effects; Cell Differentiation/drug effects/*physiology; Cell Proliferation; Compomers/*metabolism; Embryonic Stem Cells/drug effects/*physiology; Epidermal Growth Factor/pharmacology; Fibroblast Growth Factor 2/pharmacology; Flow Cytometry; Gene Expression; Homeodomain Proteins/metabolism; Humans; Induced Pluripotent Stem Cells/drug effects/*physiology; Intracellular Signaling Peptides and Proteins; Karyotype; Nanog Homeobox Protein; Nerve Tissue Proteins/genetics/metabolism; Nuclear Proteins; Octamer Transcription Factor-3/genetics/metabolism; Oligodendroglia/physiology; Proliferating Cell Nuclear Antigen/genetics/metabolism; hESC; hiPSC; human neural stem cells; neuralization; oligoprogenitors; xeno-free  
  Abstract Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) can differentiate into many cell types and are important for regenerative medicine; however, further work is needed to reliably differentiate hESC and hiPSC into neural-restricted multipotent derivatives or specialized cell types under conditions that are free from animal products. Toward this goal, we tested the transition of hESC and hiPSC lines onto xeno-free (XF) / feeder-free conditions and evaluated XF substrate preference, pluripotency, and karyotype. Critically, XF transitioned H9 hESC, Shef4 hESC, and iPS6-9 retained pluripotency (Oct-4 and NANOG), proliferation (MKI67 and PCNA), and normal karyotype. Subsequently, XF transitioned hESC and hiPSC were induced with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) to generate neuralized spheres containing primitive neural precursors, which could differentiate into astrocytes and neurons, but not oligoprogenitors. Further neuralization of spheres via LIF supplementation and attachment selection on CELLstart substrate generated adherent human neural stem cells (hNSC) with normal karyotype and high proliferation potential under XF conditions. Interestingly, adherent hNSC derived from H9, Shef4, and iPS6-9 differentiated into significant numbers of O4+ oligoprogenitors ( approximately 20-30%) with robust proliferation; however, very few GalC+ cells were observed ( approximately 2-4%), indicative of early oligodendrocytic lineage commitment. Overall, these data demonstrate the transition of multiple hESC and hiPSC lines onto XF substrate and media conditions, and a reproducible neuralization method that generated neural derivatives with multipotent cell fate potential and normal karyotype.  
  Address Physical Medicine & Rehabilitation, University of California, Irvine, California; Anatomy and Neurobiology, University of California, Irvine, California; Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, California; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, California  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0021-9967 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:24715528 Approved no  
  Call Number refbase @ user @ Serial 16836  
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Author "Karapetoff, V." openurl 
  Title The magnetic circuit Type Book Whole
  Year 1911 Publication Abbreviated Journal  
  Volume (down) 000516 Issue Pages xvii+283p  
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  Publisher "Mcgraw-Hill Book Company, Inc., New York" Place of Publication Editor  
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  Notes Approved no  
  Call Number refbase @ user @ 435 Serial 12816  
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Author DeRosa, B.A.; Van Baaren, J.M.; Dubey, G.K.; Lee, J.M.; Cuccaro, M.L.; Vance, J.M.; Pericak-Vance, M.A.; Dykxhoorn, D.M. url  doi
openurl 
  Title Derivation of autism spectrum disorder-specific induced pluripotent stem cells from peripheral blood mononuclear cells Type Journal Article
  Year 2012 Publication Neuroscience Letters Abbreviated Journal Neurosci Lett  
  Volume (down) 516 Issue 1 Pages 9-14  
  Keywords Cell Differentiation; Cells, Cultured; Child; Child Development Disorders, Pervasive/*pathology; GABAergic Neurons/*cytology; Humans; Leukocytes, Mononuclear/*cytology; Pluripotent Stem Cells; Tissue Engineering/*methods  
  Abstract Induced pluripotent stem cells (iPSCs) hold tremendous potential both as a biological tool to uncover the pathophysiology of disease by creating relevant cell models and as a source of stem cells for cell-based therapeutic applications. Typically, iPSCs have been derived by the transgenic overexpression of transcription factors associated with progenitor cell or stem cell function in fibroblasts derived from skin biopsies. However, the need for skin punch biopsies to derive fibroblasts for reprogramming can present a barrier to study participation among certain populations of individuals, including children with autism spectrum disorders (ASDs). In addition, the acquisition of skin punch biopsies in non-clinic settings presents a challenge. One potential mechanism to avoid these limitations would be the use of peripheral blood mononuclear cells (PBMCs) as the source of the cells for reprogramming. In this article we describe, for the first time, the derivation of iPSC lines from PBMCs isolated from the whole blood of autistic children, and their subsequent differentiation in GABAergic neurons.  
  Address John P. Hussman Institute for Human Genomics, University of Miami Miller School of Medicine, 1501 NW 10th Ave, BRB 509, Miami, FL 33146, USA  
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  Language English Summary Language Original Title  
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  Series Volume Series Issue Edition  
  ISSN 0304-3940 ISBN Medium  
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  Notes PMID:22405972; PMCID:PMC4278654 Approved no  
  Call Number refbase @ user @ Serial 16950  
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Author "Joshi, G.N." openurl 
  Title Indian administration Type Book Whole
  Year 1937 Publication Abbreviated Journal  
  Volume (down) 000505 Issue Pages xii+316p  
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  Publisher "Macmillan & Co Ltd, London" Place of Publication Editor  
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  Notes Approved no  
  Call Number refbase @ user @ 428 Serial 12815  
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Author Turon, X.; Tarjuelo, I.; Duran S,; Pascual, M. openurl 
  Title Characterising invasion processes with genetic data: an Atlantic clade of Clavelina lepadiformis (Ascidiacea) introduced into Mediterranean harbours Type Journal Article
  Year 2003 Publication Hydrobiologia Abbreviated Journal  
  Volume (down) 503 Issue Pages 29-35  
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  Notes Approved no  
  Call Number refbase @ user @ Serial 7985  
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