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Author Bonner, J.F.; Haas, C.J.; Fischer, I.
Title Preparation of neural stem cells and progenitors: neuronal production and grafting applications Type Journal Article
Year 2013 Publication Methods in Molecular Biology (Clifton, N.J.) Abbreviated Journal Methods Mol Biol
Volume (down) 1078 Issue Pages 65-88
Keywords Animals; Cell Culture Techniques/*methods; Cell Differentiation/drug effects; Cell Lineage/drug effects; Cell Separation/*methods; Chick Embryo; Collagenases/pharmacology; Cryopreservation; Culture Media/chemistry; Fibronectins/pharmacology; Immunohistochemistry; Laminin/pharmacology; Neural Stem Cells/*cytology/*transplantation; Neuroepithelial Cells/cytology; Neurons/*cytology; Polylysine/pharmacology; Rats; Spinal Cord/cytology; *Stem Cell Transplantation
Abstract Neural stem cells (NSC) are not only a valuable tool for the study of neural development and function, but an integral component in the development of transplantation strategies for neural disease. NSC can be used to study how neurons acquire distinct phenotypes and how the reciprocal interactions between neurons and glia in the developing nervous system shape the structure and function of the central nervous system (CNS). In addition, neurons prepared from NSC can be used to elucidate the molecular basis of neurological disorders as well as potential treatments. Although NSC can be derived from different species and many sources, including embryonic stem cells, induced pluripotent stem cells, adult CNS, and direct reprogramming of non-neural cells, isolating primary NSC directly from rat fetal tissue is the most common technique for preparation and study of neurons with a wealth of data available for comparison. Regardless of the source material, similar techniques are used to maintain NSC in culture and to differentiate NSC toward mature neural lineages. This chapter will describe specific methods for isolating multipotent NSC and neural precursor cells (NPC) from embryonic rat CNS tissue (mostly spinal cord). In particular, NPC can be separated into neuronal and glial restricted precursors (NRP and GRP, respectively) and used to reliably produce neurons or glial cells both in vitro and following transplantation into the adult CNS. This chapter will describe in detail the methods required for the isolation, propagation, storage, and differentiation of NSC and NPC isolated from rat spinal cords for subsequent in vitro or in vivo studies.
Address Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, USA
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Language English Summary Language Original Title
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Series Volume Series Issue Edition
ISSN 1064-3745 ISBN Medium
Area Expedition Conference
Notes PMID:23975822 Approved no
Call Number refbase @ user @ Serial 16877
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Author Azevedo-Pereira, R.L.; Daadi, M.M.
Title Isolation and purification of self-renewable human neural stem cells for cell therapy in experimental model of ischemic stroke Type Journal Article
Year 2013 Publication Methods in Molecular Biology (Clifton, N.J.) Abbreviated Journal Methods Mol Biol
Volume (down) 1059 Issue Pages 157-167
Keywords Animals; Cell Culture Techniques; *Cell Separation; Culture Media; Disease Models, Animal; Embryonic Stem Cells/physiology; Humans; Infarction, Middle Cerebral Artery/*therapy; Neural Stem Cells/*transplantation; Rats; Spheroids, Cellular/physiology
Abstract Human embryonic stem cells (hESCs) are pluripotent with a strong self-renewable ability making them a virtually unlimited source of neural cells for structural repair in neurological disorders. Currently, hESCs are one of the most promising cell sources amenable for commercialization of off-shelf cell therapy products. However, along with this strong proliferative capacity of hESCs comes the tumorigenic potential of these cells after transplantation. Thus, the isolation and purification of a homogeneous, population of neural stem cells (hNSCs) are of paramount importance to avoid tumor formation in the host brain. This chapter describes the isolation, neuralization, and long-term perpetuation of hNSCs derived from hESCs through use of specific mitogenic growth factors and the preparation of hNSCs for transplantation in an experimental model of stroke. Additionally, we describe methods to analyze the stroke and size of grafts using magnetic resonance imaging and Osirix software, and neuroanatomical tracing procedures to study axonal remodeling after stroke and cell transplantation.
Address Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA
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Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1064-3745 ISBN Medium
Area Expedition Conference
Notes PMID:23934842 Approved no
Call Number refbase @ user @ Serial 16878
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Author Abusalem, H.; Member, I.S.
Title Comparing Traditional FFT Based Frequency Domain Excision with Poly-Phase Transform Excision Type Journal Article
Year Publication Abbreviated Journal
Volume (down) 1024 Issue Pages 625-634
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Notes Approved no
Call Number refbase @ user @ Serial 8229
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Author Altmayer, C.
Title Real-Time Kinematic and High Accuracy Navigation With Low-Cost GPS Receivers Type Journal Article
Year 2001 Publication Most Abbreviated Journal
Volume (down) 1010 Issue January Pages 22-24
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Notes Approved no
Call Number refbase @ user @ Serial 8356
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Author Gasparovic, A.C.; Jaganjac, M.; Mihaljevic, B.; Sunjic, S.B.; Zarkovic, N.
Title Assays for the measurement of lipid peroxidation Type Journal Article
Year 2013 Publication Methods Mol Biol Abbreviated Journal
Volume (down) 965 Issue Pages 283-296
Keywords Acrolein, metabolism; Aldehydes, metabolism; Biological Markers, metabolism; Blotting, Western, methods; Cells, Cultured; Chromatography, High Pressure Liquid; Enzyme-Linked Immunosorbent Assay, methods; Immunohistochemistry, methods; Intracellular Space, metabolism; Lipid Peroxidation; Lipid Peroxides, metabolism; Malondialdehyde, metabolism; Membrane Proteins, metabolism; Oxidative Stress; Signal Transduction
Abstract Physical and emotional stress, metabolic alterations, carcinogenesis or inflammation are conditions that can trigger oxidative stress, which is defined as a balance shift of redox reactions towards oxidation, resulting in the increase of reactive oxygen species (ROS). ROS are continuously formed in small quantities during the normal metabolism of cell, however the overproduction of ROS is cytotoxic and damages macromolecules (DNA, proteins, sugars and lipids). Polyunsaturated fatty acids (PUFAs) that are esterified in membrane or storage lipids are subject to ROS-induced peroxidation resulting in the destruction of biomembranes. Final products of lipid peroxidation (LPO) are reactive aldehydes that are relatively stable and may diffuse far from the initial site of oxidative injury and act as second messengers or free radicals. The difference between physiological and pathological oxidative stress is often the occurrence of LPO and its final toxic products. In this chapter, two classes of methods for measurement of LPO are described. The first include assays for detection of LPO at the organismal level, while the second include molecular and cellular assays that reveal the mechanistic effects of LPO on the function, morphology and viability of the cells.
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Call Number refbase @ user @ Gasparovic2013 Serial 13017
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