toggle visibility Search & Display Options

Select All    Deselect All
 |   | 
Details
   print
  Records Links
Author Bouzas, A.O. url  doi
openurl 
  Title Addition theorems for spin spherical harmonics: II. Results Type Journal Article
  Year 2011 Publication Journal of Physics A: Mathematical and Theoretical Abbreviated Journal J. Phys. A: Math. Theor.  
  Volume 44 Issue 16 Pages (down) 165302  
  Keywords  
  Abstract  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1751-8113 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number refbase @ user @ Serial 12796  
Permanent link to this record
 

 
Author Alekhin, M.S.; de Haas, J.T.M.; Khodyuk, I.V.; Krämer, K.W.; Menge, P.R.; Ouspenski, V.; Dorenbos, P. openurl 
  Title Improvement of γ-ray energy resolution of LaBr3:Ce3+ scintillation detectors by Sr2+ and Ca2+ co-doping Type Journal Article
  Year 2013 Publication Appl. Phys. Lett. Abbreviated Journal  
  Volume 102 Issue Pages (down) 161915  
  Keywords  
  Abstract  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number refbase @ user @ Serial 17744  
Permanent link to this record
 

 
Author Chen, R.J.; Zhang, G.; Garfield, S.H.; Shi, Y.-J.; Chen, K.G.; Robey, P.G.; Leapman, R.D. url  doi
openurl 
  Title Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States Type Journal Article
  Year 2015 Publication PloS one Abbreviated Journal PLoS One  
  Volume 10 Issue 11 Pages (down) e0142554  
  Keywords Bone Morphogenetic Protein 4/metabolism; Cell Differentiation; Cell Line; Cell Proliferation; Glycogen/*metabolism; Glycogen Synthase/metabolism; Glycogen Synthase Kinase 3/metabolism; Humans; Induced Pluripotent Stem Cells/cytology/metabolism; Pluripotent Stem Cells/*cytology/*metabolism  
  Abstract Human pluripotent stem cells (hPSCs) represent very promising resources for cell-based regenerative medicine. It is essential to determine the biological implications of some fundamental physiological processes (such as glycogen metabolism) in these stem cells. In this report, we employ electron, immunofluorescence microscopy, and biochemical methods to study glycogen synthesis in hPSCs. Our results indicate that there is a high level of glycogen synthesis (0.28 to 0.62 mug/mug proteins) in undifferentiated human embryonic stem cells (hESCs) compared with the glycogen levels (0 to 0.25 mug/mug proteins) reported in human cancer cell lines. Moreover, we found that glycogen synthesis was regulated by bone morphogenetic protein 4 (BMP-4) and the glycogen synthase kinase 3 (GSK-3) pathway. Our observation of glycogen bodies and sustained expression of the pluripotent factor Oct-4 mediated by the potent GSK-3 inhibitor CHIR-99021 reveals an altered pluripotent state in hPSC culture. We further confirmed glycogen variations under different naive pluripotent cell growth conditions based on the addition of the GSK-3 inhibitor BIO. Our data suggest that primed hPSCs treated with naive growth conditions acquire altered pluripotent states, similar to those naive-like hPSCs, with increased glycogen synthesis. Furthermore, we found that suppression of phosphorylated glycogen synthase was an underlying mechanism responsible for altered glycogen synthesis. Thus, our novel findings regarding the dynamic changes in glycogen metabolism provide new markers to assess the energetic and various pluripotent states in hPSCs. The components of glycogen metabolic pathways offer new assays to delineate previously unrecognized properties of hPSCs under different growth conditions.  
  Address Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20892, United States of America  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1932-6203 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:26565809; PMCID:PMC4643957 Approved no  
  Call Number refbase @ user @ Serial 16722  
Permanent link to this record
 

 
Author Zhao, D.; Lin, M.; Chen, J.; Pedrosa, E.; Hrabovsky, A.; Fourcade, H.M.; Zheng, D.; Lachman, H.M. url  doi
openurl 
  Title MicroRNA Profiling of Neurons Generated Using Induced Pluripotent Stem Cells Derived from Patients with Schizophrenia and Schizoaffective Disorder, and 22q11.2 Del Type Journal Article
  Year 2015 Publication PloS one Abbreviated Journal PLoS One  
  Volume 10 Issue 7 Pages (down) e0132387  
  Keywords Adult; *Chromosome Deletion; Chromosomes, Human, Pair 22/*genetics; Female; *Gene Expression Profiling; Gene Regulatory Networks; Humans; Induced Pluripotent Stem Cells/pathology; Male; MicroRNAs/*genetics; Middle Aged; Neurons/*metabolism/pathology; Psychotic Disorders/*genetics/pathology; Schizophrenia/*genetics/pathology; Sequence Analysis, RNA  
  Abstract We are using induced pluripotent stem cell (iPSC) technology to study neuropsychiatric disorders associated with 22q11.2 microdeletions (del), the most common known schizophrenia (SZ)-associated genetic factor. Several genes in the region have been implicated; a promising candidate is DGCR8, which codes for a protein involved in microRNA (miRNA) biogenesis. We carried out miRNA expression profiling (miRNA-seq) on neurons generated from iPSCs derived from controls and SZ patients with 22q11.2 del. Using thresholds of p<0.01 for nominal significance and 1.5-fold differences in expression, 45 differentially expressed miRNAs were detected (13 lower in SZ and 32 higher). Of these, 6 were significantly down-regulated in patients after correcting for genome wide significance (FDR<0.05), including 4 miRNAs that map to the 22q11.2 del region. In addition, a nominally significant increase in the expression of several miRNAs was found in the 22q11.2 neurons that were previously found to be differentially expressed in autopsy samples and peripheral blood in SZ and autism spectrum disorders (e.g., miR-34, miR-4449, miR-146b-3p, and miR-23a-5p). Pathway and function analysis of predicted mRNA targets of the differentially expressed miRNAs showed enrichment for genes involved in neurological disease and psychological disorders for both up and down regulated miRNAs. Our findings suggest that: i. neurons with 22q11.2 del recapitulate the miRNA expression patterns expected of 22q11.2 haploinsufficiency, ii. differentially expressed miRNAs previously identified using autopsy samples and peripheral cells, both of which have significant methodological problems, are indeed disrupted in neuropsychiatric disorders and likely have an underlying genetic basis.  
  Address Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, New York, United States of America; Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, New York, United States of America; Department of Neuroscience, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, New York, United States of America; Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, New York, United States of America  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1932-6203 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:26173148; PMCID:PMC4501820 Approved no  
  Call Number refbase @ user @ Serial 16749  
Permanent link to this record
 

 
Author Chen, J.; Lin, M.; Hrabovsky, A.; Pedrosa, E.; Dean, J.; Jain, S.; Zheng, D.; Lachman, H.M. url  doi
openurl 
  Title ZNF804A Transcriptional Networks in Differentiating Neurons Derived from Induced Pluripotent Stem Cells of Human Origin Type Journal Article
  Year 2015 Publication PloS one Abbreviated Journal PLoS One  
  Volume 10 Issue 4 Pages (down) e0124597  
  Keywords *Cell Differentiation; Cell Line; Gene Knockdown Techniques; *Gene Regulatory Networks; Humans; Induced Pluripotent Stem Cells/*cytology; Kruppel-Like Transcription Factors/*genetics; Neurons/*cytology; RNA Interference; *Transcription, Genetic  
  Abstract ZNF804A (Zinc Finger Protein 804A) has been identified as a candidate gene for schizophrenia (SZ), autism spectrum disorders (ASD), and bipolar disorder (BD) in replicated genome wide association studies (GWAS) and by copy number variation (CNV) analysis. Although its function has not been well-characterized, ZNF804A contains a C2H2-type zinc-finger domain, suggesting that it has DNA binding properties, and consequently, a role in regulating gene expression. To further explore the role of ZNF804A on gene expression and its downstream targets, we used a gene knockdown (KD) approach to reduce its expression in neural progenitor cells (NPCs) derived from induced pluripotent stem cells (iPSCs). KD was accomplished by RNA interference (RNAi) using lentiviral particles containing shRNAs that target ZNF804A mRNA. Stable transduced NPC lines were generated after puromycin selection. A control cell line expressing a random (scrambled) shRNA was also generated. Neuronal differentiation was induced, RNA was harvested after 14 days and transcriptome analysis was carried out using RNA-seq. 1815 genes were found to be differentially expressed at a nominally significant level (p<0.05); 809 decreased in expression in the KD samples, while 1106 increased. Of these, 370 achieved genome wide significance (FDR<0.05); 125 were lower in the KD samples, 245 were higher. Pathway analysis showed that genes involved in interferon-signaling were enriched among those that were down-regulated in the KD samples. Correspondingly, ZNF804A KD was found to affect interferon-alpha 2 (IFNA2)-mediated gene expression. The findings suggest that ZNF804A may affect a differentiating neuron's response to inflammatory cytokines, which is consistent with models of SZ and ASD that support a role for infectious disease, and/or autoimmunity in a subgroup of patients.  
  Address Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York, United States of America; Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America; Dominick Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, United States of America; Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, United States of America  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1932-6203 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:25905630; PMCID:PMC4408091 Approved no  
  Call Number refbase @ user @ Serial 16767  
Permanent link to this record
Select All    Deselect All
 |   | 
Details
   print

Save Citations:
Export Records: