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Author (up) Azevedo-Pereira, R.L.; Daadi, M.M. url  doi
  Title Isolation and purification of self-renewable human neural stem cells for cell therapy in experimental model of ischemic stroke Type Journal Article
  Year 2013 Publication Methods in Molecular Biology (Clifton, N.J.) Abbreviated Journal Methods Mol Biol  
  Volume 1059 Issue Pages 157-167  
  Keywords Animals; Cell Culture Techniques; *Cell Separation; Culture Media; Disease Models, Animal; Embryonic Stem Cells/physiology; Humans; Infarction, Middle Cerebral Artery/*therapy; Neural Stem Cells/*transplantation; Rats; Spheroids, Cellular/physiology  
  Abstract Human embryonic stem cells (hESCs) are pluripotent with a strong self-renewable ability making them a virtually unlimited source of neural cells for structural repair in neurological disorders. Currently, hESCs are one of the most promising cell sources amenable for commercialization of off-shelf cell therapy products. However, along with this strong proliferative capacity of hESCs comes the tumorigenic potential of these cells after transplantation. Thus, the isolation and purification of a homogeneous, population of neural stem cells (hNSCs) are of paramount importance to avoid tumor formation in the host brain. This chapter describes the isolation, neuralization, and long-term perpetuation of hNSCs derived from hESCs through use of specific mitogenic growth factors and the preparation of hNSCs for transplantation in an experimental model of stroke. Additionally, we describe methods to analyze the stroke and size of grafts using magnetic resonance imaging and Osirix software, and neuroanatomical tracing procedures to study axonal remodeling after stroke and cell transplantation.  
  Address Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1064-3745 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:23934842 Approved no  
  Call Number refbase @ user @ Serial 16878  
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