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Author (up) Bonner, J.F.; Haas, C.J.; Fischer, I. url  doi
  Title Preparation of neural stem cells and progenitors: neuronal production and grafting applications Type Journal Article
  Year 2013 Publication Methods in Molecular Biology (Clifton, N.J.) Abbreviated Journal Methods Mol Biol  
  Volume 1078 Issue Pages 65-88  
  Keywords Animals; Cell Culture Techniques/*methods; Cell Differentiation/drug effects; Cell Lineage/drug effects; Cell Separation/*methods; Chick Embryo; Collagenases/pharmacology; Cryopreservation; Culture Media/chemistry; Fibronectins/pharmacology; Immunohistochemistry; Laminin/pharmacology; Neural Stem Cells/*cytology/*transplantation; Neuroepithelial Cells/cytology; Neurons/*cytology; Polylysine/pharmacology; Rats; Spinal Cord/cytology; *Stem Cell Transplantation  
  Abstract Neural stem cells (NSC) are not only a valuable tool for the study of neural development and function, but an integral component in the development of transplantation strategies for neural disease. NSC can be used to study how neurons acquire distinct phenotypes and how the reciprocal interactions between neurons and glia in the developing nervous system shape the structure and function of the central nervous system (CNS). In addition, neurons prepared from NSC can be used to elucidate the molecular basis of neurological disorders as well as potential treatments. Although NSC can be derived from different species and many sources, including embryonic stem cells, induced pluripotent stem cells, adult CNS, and direct reprogramming of non-neural cells, isolating primary NSC directly from rat fetal tissue is the most common technique for preparation and study of neurons with a wealth of data available for comparison. Regardless of the source material, similar techniques are used to maintain NSC in culture and to differentiate NSC toward mature neural lineages. This chapter will describe specific methods for isolating multipotent NSC and neural precursor cells (NPC) from embryonic rat CNS tissue (mostly spinal cord). In particular, NPC can be separated into neuronal and glial restricted precursors (NRP and GRP, respectively) and used to reliably produce neurons or glial cells both in vitro and following transplantation into the adult CNS. This chapter will describe in detail the methods required for the isolation, propagation, storage, and differentiation of NSC and NPC isolated from rat spinal cords for subsequent in vitro or in vivo studies.  
  Address Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, USA  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1064-3745 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:23975822 Approved no  
  Call Number refbase @ user @ Serial 16877  
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