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Author (up) Malik, N.; Wang, X.; Shah, S.; Efthymiou, A.G.; Yan, B.; Heman-Ackah, S.; Zhan, M.; Rao, M. url  doi
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  Title Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes Type Journal Article
  Year 2014 Publication PloS one Abbreviated Journal PLoS One  
  Volume 9 Issue 5 Pages e96139  
  Keywords Astrocytes/*metabolism; Biomarkers/metabolism; Cell Dedifferentiation; Cells, Cultured; Cerebral Cortex/cytology; Fetus/cytology; Gene Ontology; Humans; Induced Pluripotent Stem Cells/*metabolism; Neural Stem Cells/physiology; Oligonucleotide Array Sequence Analysis; Signal Transduction; Transcription Factors/*genetics/metabolism; *Transcriptome; Up-Regulation  
  Abstract Astrocytes are the most abundant cell type in the central nervous system (CNS) and have a multitude of functions that include maintenance of CNS homeostasis, trophic support of neurons, detoxification, and immune surveillance. It has only recently been appreciated that astrocyte dysfunction is a primary cause of many neurological disorders. Despite their importance in disease very little is known about global gene expression for human astrocytes. We have performed a microarray expression analysis of human fetal astrocytes to identify genes and signaling pathways that are important for astrocyte development and maintenance. Our analysis confirmed that the fetal astrocytes express high levels of the core astrocyte marker GFAP and the transcription factors from the NFI family which have been shown to play important roles in astrocyte development. A group of novel markers were identified that distinguish fetal astrocytes from pluripotent stem cell-derived neural stem cells (NSCs) and NSC-derived neurons. As in murine astrocytes, the Notch signaling pathway appears to be particularly important for cell fate decisions between the astrocyte and neuronal lineages in human astrocytes. These findings unveil the repertoire of genes expressed in human astrocytes and serve as a basis for further studies to better understand astrocyte biology, especially as it relates to disease.  
  Address National Institutes of Health, NIAMS, Bethesda, Maryland, United States of America; National Institutes of Health, NIH Center for Regenerative Medicine, Bethesda, Maryland, United States of America  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1932-6203 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:24848099; PMCID:PMC4029581 Approved no  
  Call Number refbase @ user @ Serial 16827  
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