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Author (up) Juan, A.H.; Wang, S.; Ko, K.D.; Zare, H.; Tsai, P.-F.; Feng, X.; Vivanco, K.O.; Ascoli, A.M.; Gutierrez-Cruz, G.; Krebs, J.; Sidoli, S.; Knight, A.L.; Pedersen, R.A.; Garcia, B.A.; Casellas, R.; Zou, J.; Sartorelli, V. url  doi
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  Title Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells Type Journal Article
  Year 2016 Publication Cell Reports Abbreviated Journal Cell Rep  
  Volume 17 Issue 5 Pages 1369-1382  
  Keywords H3K27 methylation; embryonic stem cells; polycomb proteins  
  Abstract The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.  
  Address Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: sartorev@mail.nih.gov  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2211-1247 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:27783950; PMCID:PMC5123747 Approved no  
  Call Number refbase @ user @ Serial 16650  
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