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Author |
Hulatt, C.J.; Thomas, D.N. |

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Title |
Energy efficiency of an outdoor microalgal photobioreactor sited at mid-temperate latitude |
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Journal Article |
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Year |
2011 |
Publication |
Bioresource Technology |
Abbreviated Journal |
Bioresour Technol |
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Volume |
102 |
Issue |
12 |
Pages |
6687-6695 |
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Keywords |
Biomass; *Bioreactors; Climate; Geography; Microalgae/growth & development/*metabolism; Oxygen/metabolism; Scenedesmus/growth & development/*metabolism; Seasons; Solar Energy |
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Abstract |
This work examined the energetic performance of a 6-month semi-continuous cultivation of Scenedesmus obliquus in an outdoor photobioreactor at mid-temperate latitude, without temperature control. By measuring the seasonal biomass production (mean 11.31, range 1.39-23.67 g m(-2)d(-1)), higher heating value (22.94 kJ g(-1)) and solar irradiance, the mean seasonally-averaged photosynthetic efficiency (2.18%) and gross energy productivity (0.27 MJ m(-2) d(-1)) was calculated. When comparing the solar energy conversion efficiency to the energy investment for culture circulation, significant improvements in reactor energy input must be made to make the system viable. Using the data collected to model the energetic performance of a substitute photobioreactor design, we conclude that sustainable photobioreactor cultivation of microalgae in similar temperate climates requires a short light path and low power input, only reasonably obtained by flat-panel systems. However, temperature control was not necessary for effective long-term cultivation. |
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School of Ocean Sciences, College of Natural Sciences, Bangor University, Askew Street, Menai Bridge, Anglesey LL59 5AB, UK. osp418@bangor.ac.uk |
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0960-8524 |
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PMID:21511466 |
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refbase @ user @ |
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12984 |
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Author |
Lakaniemi, A.-M.; Hulatt, C.J.; Wakeman, K.D.; Thomas, D.N.; Puhakka, J.A. |

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Title |
Eukaryotic and prokaryotic microbial communities during microalgal biomass production |
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Journal Article |
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Year |
2012 |
Publication |
Bioresource Technology |
Abbreviated Journal |
Bioresour Technol |
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Volume |
124 |
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Pages |
387-393 |
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Keywords |
Bacteria/classification/genetics/metabolism; *Biomass; Electrophoresis, Polyacrylamide Gel; Eukaryotic Cells; Microalgae/*metabolism; Phylogeny; Polymerase Chain Reaction; Prokaryotic Cells |
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Eukaryotic and bacterial communities were characterized and quantified in microalgal photobioreactor cultures of freshwater Chlorella vulgaris and marine Dunaliella tertiolecta. The microalgae exhibited good growth, whilst both cultures contained diverse bacterial communities. Both cultures included Proteobacteria and Bacteroidetes, while C. vulgaris cultures also contained Actinobacteria. The bacterial genera present in the cultures were different due to different growth medium salinities and possibly different extracellular products. Bacterial community profiles were relatively stable in D. tertiolecta cultures but not in C. vulgaris cultures likely due to presence of ciliates (Colpoda sp.) in the latter. The presence of ciliates did not, however, cause decrease in total number of C. vulgaris or bacteria during 14 days of cultivation. Quantitative PCR (qPCR) reliably showed relative microalgal and bacterial cell numbers in the batch cultures with stable microbial communities, but was not effective when bacterial communities varied. Raw culture samples were successfully used as qPCR templates. |
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Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland. aino-maija.lakaniemi@tut.fi |
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0960-8524 |
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PMID:22995170 |
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refbase @ user @ |
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12987 |
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Author |
Thomas, D.N. |

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Title |
Photosynthetic microbes in freezing deserts |
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Journal Article |
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2005 |
Publication |
Trends in Microbiology |
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Trends Microbiol |
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13 |
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3 |
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87-88 |
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Polar deserts are not devoid of life despite the extreme low temperature and scarcity of water. Recently, patterned stone fields – caused by periglacial activity – have been surveyed in the Arctic and Antarctic. It was found that the productivity of the cyanobacteria and algae (hypoliths) that colonise the underside of the stones is strongly related to the pattern of the stones. The hypolith assemblages were in some cases as productive as lichens, bryophytes and plants that resided nearby. |
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Elsevier Science B.V. |
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Amsterdam |
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English |
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English |
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0966-842X |
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refbase @ admin @ Thomas2005 |
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755 |
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Author |
Gomez, I.; Wiencke, C.; Thomas, D.N. |

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Title |
Variations in photosynthetic characteristics of the Antarctic marine brown alga Ascoseira mirabilis in relation to thallus age and size |
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Journal Article |
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Year |
1996 |
Publication |
European Journal of Phycology |
Abbreviated Journal |
Eur J Phycol |
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31 |
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2 |
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167-172 |
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Keywords |
photosynthesis: thallus: carbon fixation; growth curves; algae; age; Thalli; marine flora; Ascoseira mirabilis; Antarctica |
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Growth, photosynthesis, dark respiration, chlorophyll a (Chl a) content and dry weight were measured in 2- and 3-year-old plants of Ascoseira mirabilis (Ascoseirales), cultivated in the laboratory under changing daylengths which matched the seasonal variations in the Antarctic. Determinations were made in four thallus regions. Growth of A. mirabilis was seasonal, with higher rates in spring. Parameters such as net photosynthesis (P sub(max)), photosynthetic efficiency ( alpha ), both measured on a fresh weight (FW) basis, and dry weight content, showed significant age- and size-dependent variations. In contrast, no variations were observed in dark respiration, initial light-saturating point of photosynthesis (I sub(k)) and Chl a contents. P sub(max) had maximum values close to 16.5 mu mol O sub(2)/g super(1) FW/h in 2-year-old plants, whereas in 3-year-old plants maximum values of 8 mu mol O sub(2)/g FW/h were determined. The alpha -values reached maximum rates of 1.4 and 0.6 mu mol O sub(2)/g FW/h/( mu mol photons/m super(2)/s) in 2- and 3-year-old plants, respectively. Light compensation point (I sub(c)), dry weight ratios and Chl a contents varied significantly along the length of the blade. Maximum dry:fresh weight ratios were observed in the basal region, with values close to 18%. Distal regions of the 3-year-old plants had significantly higher dry weight content than 2-year-old plants (17.5% and 13%, respectively). Chl a concentrations increased towards the middle regions of the thallus to values close to 0.35 mg Chl a/g FW. The results indicate that some morpho-functional processes in A. mirabilis, especially net photosynthesis and photosynthetic efficiency, are governed by age of the plant, thereby reflecting differences in biomass allocation and size. Our data also confirm the previously demonstrated relationship between growth and seasonal physiological activity that allows A. mirabilis to survive under the low light conditions prevailing in the Antarctic. |
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0967-0262 |
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growth curves; size; Thalli; marine flora; thallus |
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Call Number |
refbase @ admin @ Gomez++1996 |
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737 |
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Author |
Mock, T.; Thomas, D.N. |

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Title |
Recent advances in sea-ice microbiology |
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Journal Article |
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Year |
2005 |
Publication |
Environmental Microbiology |
Abbreviated Journal |
Environ Microbiol |
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7 |
Issue |
5 |
Pages |
605-619 |
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Over the past 50 years there has been much effort invested in the investigation of the ecology of sea ice. Sea ice is an ephemeral feature of the Arctic and Southern Oceans and smaller water bodies such as the Baltic and Caspian Seas. The semisolid ice matrix provides a range of habitats in which a diverse range of microbial organisms thrive. In the past 5 years there has been considerable steps forward in sea-ice research, in particular regarding the analysis of sea-ice microstructure and the investigation of the diversity and adaptation of microbial communities. These studies include: (i) controlled simulated and in situ studies on a micrometer scale to unravel the dynamic of the microhabitat with consequences for the organisms; (ii) the introduction of molecular approaches to uncover the diversity of uncultured still unknown microorganisms; and (iii) studies into the molecular adaptation of selected model organisms to the extreme environment. This minireview presents some of the most recent findings from sea-ice studies within the framework of these aims. |
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Blackwell Publishing, Inc. |
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Oxford |
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1462-2912 |
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Minireview |
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Call Number |
refbase @ admin @ Mock+Thomas2005 |
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750 |
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